Application
Western blot
Sample
Apteronotus leptorhynchus Tissue lysate - whole (Brain)
Loading amount
50 µg
Specification
Brain
Gel Running Conditions
Reduced Denaturing (4-15%)
Blocking step
Milk as blocking agent for 15 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Other product details
Dilution
1/500
Incubation time
1 hour(s) and 30 minute(s) · Temperature: 24°C · Diluent: 2.5% milk in PBS-Tween20
Secondary antibody
Name
Non-Abcam antibody was used: Alexa Fluor 488-conjugated goat anti-rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/1000
Detection
Detection method
Fluorescence detection
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 65, 130 kDa
Additional data
Additional Notes
The antibody detects specific bands, but the molecular weights are much higher than predicted. These may represent isoforms found in this species.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jan 23 2013