Anti-Sohlh2 抗体 (ab101402)
Key features and details
- Rabbit polyclonal to Sohlh2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Sohlh2 antibody -
製品の詳細
Rabbit polyclonal to Sohlh2 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Recombinant protein fragment containing a sequence corresponding to a region within amino acids 45-198 of Human Sohlh2 (AAH25383; UniProt ID: Q9NX45 isoform 2).
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ポジティブ・コントロール
- NT2D1, IMR32, U-87MG and MCF7 cell lysates
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab101402の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 25 kDa.
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IHC-P | (1) |
1/100 - 1/500. Suggested antigen retrieval: use heat mediated 10 mM Citrate buffer or Tris EDTA buffer (pH8.0).
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特記事項 |
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WB
1/500 - 1/3000. Predicted molecular weight: 25 kDa. |
IHC-P
1/100 - 1/500. Suggested antigen retrieval: use heat mediated 10 mM Citrate buffer or Tris EDTA buffer (pH8.0). |
ターゲット情報
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機能
Probable transcription factor, which may be involved in spermatogenesis and oogenesis. -
配列類似性
Contains 1 bHLH (basic helix-loop-helix) domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 100526761 Human
- Entrez Gene: 54937 Human
- SwissProt: Q9NX45 Human
- Unigene: 124519 Human
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別名
- bHLHe81 antibody
- FLJ20449 antibody
- FLJ57222 antibody
see all
画像
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All lanes : Anti-Sohlh2 antibody (ab101402) at 1/1000 dilution
Lane 1 : NT2D1 whole cell lysate
Lane 2 : MCF7 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 25 kDa
12% SDS PAGE -
Immunohistochemical analysis of paraffin embedded Ca922 xenograft, using ab101402 at a 1/500 dilution.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab101402 は 3 報の論文で使用されています。
- Ji S et al. Sohlh2 suppresses epithelial to mesenchymal transition in breast cancer via downregulation of IL-8. Oncotarget 7:49411-49424 (2016). PubMed: 27384482
- Zhang H et al. Sohlh2 inhibits human ovarian cancer cell invasion and metastasis by transcriptional inactivation of MMP9. Mol Carcinog 55:1127-37 (2016). PubMed: 26153894
- Zhang H et al. Sohlh2 inhibits ovarian cancer cell proliferation by upregulation of p21 and downregulation of cyclin D1. Carcinogenesis 35:1863-71 (2014). PubMed: 24858206