製品の概要

  • 製品名
    Anti-Sohlh1 antibody
  • 製品の詳細
    Rabbit polyclonal to Sohlh1
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Mouse
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Mouse Sohlh1.

    Immunogen の所有権に関して

    (Peptide available as ab42012.)

  • ポジティブ･コントロール
    • This antibody gave a positive signal in the following Mouse Tissue lysates: Fetus (14 Day Old), Ovary (data not shown)

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab41520 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 2 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).

ターゲット情報

  • 機能
    Probable transcription factor required during spermatogenesis and oogenesis.
  • 関連疾患
    Note=Genetic variations in SOHLH1 may be associated with non-obstructive azoospermia.
  • 配列類似性
    Contains 1 basic helix-loop-helix (bHLH) domain.
  • 細胞内局在
    Cytoplasm. Nucleus.
  • Information by UniProt
  • 参照データベース
    • 別名
      • GM110 antibody
      • Helix Loop Helix Protein TOHLH1 antibody
      • Sohlh1 antibody
      • SOLH1_HUMAN antibody
      • Spermatogenesis- and oogenesis-specific basic helix-loop-helix-containing protein 1 antibody
      • TOHLH1 antibody
      see all

    画像

    • Western blot - Anti-Sohlh1 antibody (ab41520)
      Western blot - Anti-Sohlh1 antibody (ab41520)
      Anti-Sohlh1 antibody (ab41520) at 2 µg/ml + Mouse Fetus (14 Day Old) Tissue Lysate at 20 µg

      Secondary
      Goat polyclonal to rabbit IgG - H&L - Pre Adsorbed (HRP) at 1/3000 dilution

      Performed under reducing conditions.

      Predicted band size: 38 kDa
      Observed band size: 38 kDa
      Additional bands at: 130 kDa, 73 kDa, 85 kDa. We are unsure as to the identity of these extra bands.

    • Immunocytochemistry/ Immunofluorescence - Anti-Sohlh1 antibody (ab41520)
      Immunocytochemistry/ Immunofluorescence - Anti-Sohlh1 antibody (ab41520)Image courtesy of an anonymous Abreview.
      ab41520 staining Sohlh1 in in vitro cultures of murine spermatogonia by Immunocytochemistry/ Immunofluorescence.
      Cells were fixed in paraformaldehyde, permeabilized using 0.1% Triton X-100, blocked with a blocking agent for 1 hour at 25°C and then incubated with ab41520 at a 1/50 dilution for 20 hours at 4°C. The secondary used was an Alexa-Fluor 488 conjugated chicken anti-rabbit polyclonal, used at a 1/500 dilution.

      See Abreview

    参考文献

    This product has been referenced in:
    • Desimio MG  et al. SOHLH1 and SOHLH2 directly down-regulate STIMULATED BY RETINOIC ACID 8 (STRA8) expression. Cell Cycle 14:1036-45 (2015). Read more (PubMed: 25603532) »

    See 1 Publication for this product

    ab41520 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    レビューと Q&A

    レビューを送る 質問を送る

    3 reviews or Q&A

    10
    Votes: Highest First

    Immunohistochemistry (Frozen sections) abreview for Anti-Sohlh1 antibody

     Poor
    Abreviews
    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Testis)
    Permeabilization
    Yes - 0.1% Triton X-100 in PBS
    Specification
    Testis
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Read More
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    Username

    Mr. Bryan Niedenberger

    Verified customer

    投稿 Apr 07 2016

    Immunohistochemistry (Frozen sections) abreview for Anti-Sohlh1 antibody

     Poor
    Abreviews
    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (testis)
    Permeabilization
    No
    Specification
    testis
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More
    abreview image
    Username

    Abcam user community

    Verified customer

    投稿 Feb 25 2015

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Sohlh1 antibody

     Good
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (In vitro cultures of spermatogonia)
    Specification
    In vitro cultures of spermatogonia
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - PBS + 0.1% Triton-X 100
    Blocking step
    1X Roche Blocking Reagent Cat. No. 11 096 176 001 as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Read More
    abreview image
    Username

    Abcam user community

    Verified customer

    投稿 Sep 21 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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