Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue lysate - other (canine myocardial infarct)
Specification
canine myocardial infarct
Antigen retrieval step
None
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 2%
Other product details
Dilution
1/20
Incubation time
2 hour(s) and 0 minute(s)
Secondary antibody
Name
Non-Abcam antibody was used: Vectastain anti mouse IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Additional data
PubMed ID
15258177: View
Additional Notes
Expression of smoothelin in canine epicardial coronary artery (A) and in the normal
cardiac vasculature (B). Coronary smooth muscle cells stained intensely smoothelin (A). Smoothelin (B) expression was dependent on the size of the vessel. Larger arterioles (B) were more likely to express desmin than smaller ones. Pericytes coating capillaries didn't stain for smoothelin (B). Counterstained
with eosin.
Tissue fix B*5 without formaldehyde.
HRP activity detected with DAB+Ni
In sample selection there is now tissue option?
cardiac vasculature (B). Coronary smooth muscle cells stained intensely smoothelin (A). Smoothelin (B) expression was dependent on the size of the vessel. Larger arterioles (B) were more likely to express desmin than smaller ones. Pericytes coating capillaries didn't stain for smoothelin (B). Counterstained
with eosin.
Tissue fix B*5 without formaldehyde.
HRP activity detected with DAB+Ni
In sample selection there is now tissue option?
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Marcin Dobaczewski
Verified customer
投稿 Aug 11 2005