Application
Western blot
Sample
Human Cell lysate - nuclear (hESC)
Loading amount
20 µg
Specification
hESC
Treatment
BMP4 for 24h
Gel Running Conditions
Reduced Denaturing (4-12% gradient)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Other product details
Dilution
1/3000
Incubation time
11 hour(s) and 0 minute(s) · Temperature: 23°C · Diluent: Milk
Secondary antibody
Name
Non-Abcam antibody was used:
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/20000
Detection
Detection method
ECL+
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 56 kDa
Negative control
Cytoplasmic extract
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Aug 15 2008