Application
Western blot
Loading amount
60 µg
Gel Running Conditions
Reduced Non-Denaturing (Native) (12)
Sample
Mouse Tissue lysate - whole (Mouse gastrocnemius)
Specification
Mouse gastrocnemius
Blocking step
Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 26°C
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 26°C · Diluent: Odyssey Blocking Buffer + 0.2% tween
Dilution
1/5000
Secondary antibody
Name
Non-Abcam antibody was used: IRD 800 CW Goat Anti rabbit (H+L)
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye 800CW
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye 800CW
Dilution
1/15000
Detection
Detection method
Infrared
Bands
Specific: 48-52 kDa
Exposure
10 minute(s) and 0 second(s)
Additional data
Additional Notes
Antibody tested on Infrared Technology with no stimulation. Red Channel = GAPDH loading control, green channel Anti-Smad3 (phospho S423 + S425) antibody (ab51451). Anti-Smad3 (phospho S423 + S425) [EP823Y] antibody (ab52903) has also been tested for Infrared technology (see related abreview) but it didn't work. Therefore, recommend the ab51451 for Infrared Odyssey technology.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Francesco Elia Marino
Verified customer
投稿 Mar 26 2014