Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (hypothalamus PVN sections)
Specification
hypothalamus PVN sections
Fixative
70% ethanol
Permeabilization
Yes - triton-x
Blocking step
2% BSA 2% NGS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C
Other product details
Dilution
1/200
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: 2% BSA 2% NGS in PBS
Secondary antibody
Name
Non-Abcam antibody was used: goat anti-rabbit Alexa-488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/500
Additional data
Additional Notes
Highly specific staining for sigma-1 receptor in the PVN of the hypothalamus in frozen 12um sections. Optimisation using concentrations of primary antibody 1:1000-1:200. No other fixatives used. Negative control (no primary antibody) showed no background fluorescence
Counter stain propidium iodide for nuclei
Left panel: Sigma1R-Alexa488, right panel: Negative control.
Counter stain propidium iodide for nuclei
Left panel: Sigma1R-Alexa488, right panel: Negative control.
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投稿 Oct 05 2011