Application
Western blot
Sample
Human Cell lysate - whole cell (Various cell lines (amniocyte, HeLa, 293A, etc))
Loading amount
60 µg
Specification
Various cell lines (amniocyte, HeLa, 293A, etc)
Gel Running Conditions
Non-reduced Denaturing (8% gel)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/3000
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% skim milk
Secondary antibody
Name
Non-Abcam antibody was used: Anti-mouse
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/3000
Detection
Detection method
ECL
Exposure
20 second(s)
Bands
Specific: 68 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Ms. Noori Lee
Verified customer
投稿 Aug 07 2009