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Products:Immunology >> Immunoglobulins >> Heavy Chain >> IgG
Overview
Product name
Donkey anti-Sheep IgG H&L (PE) secondary antibody
IgG 抗体 (1693件) 一覧
Description
Donkey F(ab')2 polyclonal Secondary Antibody to Sheep IgG - H&L (PE), pre-adsorbed
Target species
Sheep
Tested applications
ICC/IF, IHC-P, IHC-Fr, Immunomicroscopy, Flow Cytmore details
Minimal
cross-reactivity
Chicken, Guinea pig, Hamster, Horse, Human, Mouse, Rabbit, Ratmore details
Immunogen
Sheep IgG whole molecule
Properties
Form
Liquid
Storage instructions
Store at +4°C in the dark. Do not freeze.
Storage buffer
0.01M Na phosphate, 0.25M NaCl, pH7.6, 0.05% Na azide, 0.01% Thimerisol, 15mg/ml BSA
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Prepared from monospecific antiserum by immunoaffinity chromatography using sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation
Conjugation notes
R-Phycoerythrin (R-PE) Molecular Weight 240,000 daltons) Absorption Wavelength: 488 nm Emission Wavelength: 575 nm OD566nm/OD280nm: 2.8
Clonality
Polyclonal
Research areas
Secondary antibodies >> anti-Sheep >> IgG >> Fluorophore >> Phycoerythrin
Immunology >> Immunoglobulins >> Heavy Chain >> IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab7009 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF
ICC/IF: 1/100 - 1/250.ICC/IF: 1/100 - 1/250.
IHC-P
IHC-P: 1/50 - 1/200.IHC-P: 1/50 - 1/200.
IHC-Fr
IHC-Fr: 1/50 - 1/200.IHC-Fr: 1/50 - 1/200.
Immunomicroscopy
IM: 1/50 - 1/200.IM: 1/50 - 1/200.
Flow Cyt
Flow Cyt: 1/100 - 1/250.Flow Cyt: 1/100 - 1/250.
References for Donkey anti-Sheep IgG H&L (PE) secondary antibody (ab7009)
This product has been referenced in:
- Tanaka Yet al. Sustained macroscopic engraftment of cynomolgus embryonic stem cells in xenogeneic large animals after in utero transplantation. Stem Cells Dev 17:367-81 (2008). Flow Cyt.Read more (PubMed: 18447651) »
See 1 publication for this product
Publishing research using ab7009? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"