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Product Name
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Donkey F(ab')2 polyclonal Secondary Antibody to Sheep IgG - H&L (PE), pre-adsorbed
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Product type
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Secondary antibodies
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Description
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Donkey F(ab')2 polyclonal Secondary Antibody to Sheep IgG - H&L (PE), pre-adsorbed
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Immunogen
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Sheep IgG whole molecule
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Tested applications
(see key)
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Flow Cyt, IHC-Fr, IHC-P, IM
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Application notes
(see key)
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Recommended dilutions
Flow Cyt: Use at an assay dependent concentration.
IHC-P: Use at an assay dependent concentration.
IHC-Fr: Use at an assay dependent concentration.
IM: Use at an assay dependent concentration.
As a general guideline, dilutions of 1:50 to 1:200 should be suitable for most applications.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Please refer to the relevant conjugation for dilutions specific for this product:
Conjugate |
ELISA |
Blotting |
Flow Cytometry |
IHC |
Fluorescein |
nd |
nd |
50 - 200 |
200 - 1,000 |
Rhodamine, Texas RedTM |
nd |
nd |
50 - 200 |
200 - 1,000 |
R-Phycoerythrin |
nd |
nd |
100 - 200 |
nd |
Cyanines |
nd |
nd |
400 |
100 - 1,000 |
Biotin |
5,000 - 20,000 |
3,000 - 10,000 |
nd |
100 - 1,000 |
Peroxidase |
4,000 - 10,000 |
2,000 - 5,000 |
nd |
200 - 2,000 |
Alkaline Phosphatase |
1,000 - 2,000 |
500 - 1,000 |
nd |
100 - 1,000 |
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Research areas
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Secondary antibodies >> anti-Sheep >> IgG >> Fluorophore >> Phycoerythrin
Immunology >> Immunoglobulins >> Heavy Chain >> IgG
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Donkey F(ab')2 polyclonal Secondary Antibody to Sheep IgG - H&L (PE), pre-adsorbed (ab7009)の別名 |
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Raised in
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Donkey
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Clonality
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Polyclonal
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Purity
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Immunogen affinity purified
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Conjugation notes
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R-Phycoerythrin (R-PE) Molecular Weight 240,000 daltons)
Absorption Wavelength: 488 nm
Emission Wavelength: 575 nm
OD566nm/OD280nm: 2.8
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Storage buffer
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0.01M Na phosphate, 0.25M NaCl, pH7.6, 0.05% Na azide, 0.01% Thimerisol, 15mg/ml BSA Material safety datasheet (MSDS) for this product:
Sodium Phosphate MSDS
この製品は、毒物及び劇物取締法に基づく「毒物」に該当します。
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Purification notes
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Prepared from monospecific antiserum by immunoaffinity chromatography using sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation
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Form
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Liquid
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Conjugation
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Phycoerythrin
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Concentration
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0.500 mg/ml
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Storage instructions
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Store at +4°C in the dark. Do not freeze.
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Abcam では全ての製品情報を一元管理しており、 Donkey F(ab')2 polyclonal Secondary Antibody to Sheep IgG - H&L (PE), pre-adsorbed について私たちが知りうる全ての情報がこのデータシートでご覧いただけます。ご不明な点がありましたら、 お問い合わせください。 |
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References for Donkey F(ab')2 polyclonal Secondary Antibody to Sheep IgG - H&L (PE), pre-adsorbed (ab7009)
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This product has been used in:
Tanaka Y et al. Sustained macroscopic engraftment of cynomolgus embryonic stem cells in xenogeneic large animals after in utero transplantation. Stem Cells Dev 17:367-81 (2008). Flow Cyt. PubMed: 18447651
If you publish research using ab7009 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to IgG
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Donkey F(ab')2 polyclonal Secondary Antibody to Sheep IgG - H&L (PE), pre-adsorbed - more information
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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