Donkey anti-Sheep IgG H&L (PE) secondary antibody (ab7009)

Thank you for your response, please see below the response from the customer;

I thought I had explained myself clearly. I am well aware that these are donkey derived antibodies! I am using them against sheeppolyclonal antibodies (purified to contain onlyIgG). The sheep polyclonals are just attached to beads. The beads are then measured for PE fluorescence. The fact is that the same product made byanother company seems to work quite well whereas the Abcam one does not.

I'm not sure what other information I can give, but I'm pretty disappointed with that response from Abcam.

Kind regards,

ANSWER:

Thank you for your response and the further clarification.

I am afraid this information was not provided in the initial e-mail.

Your credit note ID is 20806.

I am sorry that this antibody did not perform as stated on the datasheet, I have asked our Finance department to issue a credit note for you. The credit note may be used in one of the following ways:

(1) Redeemed against the original invoice if this hasn't already been paid.
(2) Held on the account for use against a future order.
(3) A full refund can be offered where no other invoices are outstanding.

Please contact your Finance department to confirm how you would like the credit note to be used and ensure it is not redeemed without your knowledge.

To specifically receive a refund please ask your Finance department to contact our Finance department at creditcontrol@abcam.com or by telephone using the information at the “Contact Us” link in the top right corner of our website.

The credit note ID is for your reference only, please refer to the credit note ID in any correspondence with our accounting department. We will send you the completed credit note by email or postal mail with the actual credit note number which will start with the letters CGB.

I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service should you require further expert advice

My apologies! Sorry that was pretty silly of me to forget the catalogue number .. K


It is ab7009 Donkey F(ab')2 polyclonal Secondary Antibody to Sheep IgG - H&L (PE), pre-adsorbed

Please see below for an email we received from a customer;

I have tested this product (twice) and was disappointed with the results. When compared to a 4 year old vial of the equivalent Santa Cruz product, the Abcam antibody produced about 1/10th the signal. The application was a validation of sheep polyclonal antibody coupled beads, used for and read by a Bio-plex instrument (basically measuring the amount of sheep antibodies bound to the beads). All conditions in the experiment were the same, including test sample and antibody concentration.


Lot #: GR38595

Sapphire order number: PO-13364
Abcam invoice number : 1315698B



Sorry again!



Hope that helps!



Kind regards,

ANSWER:

My colleague is out of office and she has asked me to look after her customers in her absence.

Thank you for response and for confirming the product code, the batch and the order number> I am a bit confused about this enquiry since ab7009 is a donkey polyclonal antibody and not sheep, so it contains donkey IgG (F(ab')2) - fragment.

It was raised against sheep IgG so the immunogne is sheep IgG whole molecule but the actual product consists of donkey immunoglobulins.

Furthermore, ab7009 has not been tested in IP or any separation technique which requires beads. It has been characterized ICC/IF, IHC-P, IHC-Fr, Immunomicroscopy, Flow Cyt.

If you need any further assistance in the future, please do not hesitate to contact me.

Antibody seems not to perform recognizing serum opsonised beads or lateral flow capture lines that get a strong signal when using monoclonal anti sheep FITC antibody. Antibodies in lateral flow were also not recognised by anti-lambda ovine antibody so potentially the polyclonal recognises preferentially lambda which would be correct as sheep have 99% lambda antibodies. It would be worthwhile testing this out and put a warning onto the use of the antibody.

ANSWER:

Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody in lateral flow experiments.

We appreciate your valuable feedback. Regrettably, this antibody has not been tested in lateral flow, so we would unfortunately not be able to guarantee how well it will work in this case.

I would like to investigate this particular case for you, and also obtain more information for our quality monitoring records. In order to proceed with this, I would appreciate if you are able to provide some further information on the experiments:

1. Please provide more detail regarding what type of samples were tested?
2. What was the protocol used?
3. I would appreciate if you could also provide any images which would help us to assess the results.

Also, could you confirm if the antibody has been tried in a tested application? I would like to reassure you that this antibody is tested and covered by our 6 month guarantee for ICC/IF, IHC-P, IHC-Fr, Immunomicroscopy, Flow Cytometry. In the event that a product is not functioning in the tested applications and species cited on the product data sheet (and the problem has been reported within 6 months of purchase), we will be pleased to provide a credit note, free of charge replacement or refund.

Thank you for your time and cooperation. We look forward to receiving the requested information.