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Products:Immunology >> Immunoglobulins >> Heavy Chain >> IgG
Overview
Product name
Donkey anti-Sheep IgG H&L (Biotin) secondary antibody
IgG 抗体 (1691件) 一覧
Description
Donkey polyclonal Secondary Antibody to Sheep IgG - H&L (Biotin)
Target species
Sheep
Tested applications
ICC/IF, IHC-P, IHC-Fr, WB, ELISA, Immunomicroscopy, Dot Blotmore details
Immunogen
Full length native Sheep IgG (purified).
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.01% Sodium Azide
Constituents: 10mg/ml BSA, 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
Concentration
Concentration information loading...
Purity
IgG fraction
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities
Conjugation notes
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule
Clonality
Polyclonal
Research areas
Secondary antibodies >> anti-Sheep >> IgG >> Biotin
Immunology >> Immunoglobulins >> Heavy Chain >> IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab6899 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF
IHC-P
IHC-Fr
WB
ELISA
Immunomicroscopy
Dot Blot
Application notes
Dot: Use at an assay dependant dilution.
ELISA: 1/30000 - 1/130000.
IHC-P: Use at an assay dependant dilution.
IHC-Fr: Use at an assay dependant dilution.
IM: Use at an assay dependant dilution.
WB: Use at an assay dependant dilution.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
References for Donkey anti-Sheep IgG H&L (Biotin) secondary antibody (ab6899)
ab6899 has not yet been referenced specifically in any publications.
Publishing research using ab6899? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"