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Product Name
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Rabbit polyclonal Secondary Antibody to Sheep IgG - H&L (AP)
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Product type
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Secondary antibodies
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Description
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Rabbit polyclonal Secondary Antibody to Sheep IgG - H&L (AP)
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Immunogen
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Sheep IgG whole molecule
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Tested applications
(see key)
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Dot, ELISA, IHC-Fr, IHC-P, IM, WB
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Application notes
(see key)
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Recommended dilutions
suitable for immunoblotting (western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency
Please refer to the relevant conjugation for dilutions specific for this product:
Conjugate |
ELISA |
Blotting |
Flow Cytometry |
IHC |
Fluorescein |
nd |
nd |
50 - 200 |
200 - 1,000 |
Rhodamine, Texas RedTM |
nd |
nd |
50 - 200 |
200 - 1,000 |
R-Phycoerythrin |
nd |
nd |
100 - 200 |
nd |
Cyanines |
nd |
nd |
400 |
100 - 1,000 |
Biotin |
5,000 - 20,000 |
3,000 - 10,000 |
nd |
100 - 1,000 |
Peroxidase |
4,000 - 10,000 |
2,000 - 5,000 |
nd |
200 - 2,000 |
Alkaline Phosphatase |
1,000 - 2,000 |
500 - 1,000 |
nd |
100 - 1,000 |
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Research areas
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Secondary antibodies >> anti-Sheep >> IgG >> Enzyme >> Alkaline Phos
Immunology >> Immunoglobulins >> Heavy Chain >> IgG
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Rabbit polyclonal Secondary Antibody to Sheep IgG - H&L (AP) (ab6748)の別名 |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Purity
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IgG fraction
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Conjugation notes
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This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads
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Storage buffer
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Preservative: 0.05% Sodium Azide
Constituents: 15mg/ml BSA, 0.25M Sodium chloride, 0.01M Sodium phosphate, 0.01% Thimerisol, pH 7.6 Material safety datasheets (MSDS) for this product:
Sodium Azide MSDS
Sodium Phosphate MSDS
この製品は、毒物及び劇物取締法に基づく「毒物」に該当します。
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Purification notes
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Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons).
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Form
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Liquid
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Conjugation
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Alkaline Phosphatase
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C. Do not freeze.
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Abcam では全ての製品情報を一元管理しており、 Rabbit polyclonal Secondary Antibody to Sheep IgG - H&L (AP) について私たちが知りうる全ての情報がこのデータシートでご覧いただけます。ご不明な点がありましたら、 お問い合わせください。 |
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References for Rabbit polyclonal Secondary Antibody to Sheep IgG - H&L (AP) (ab6748)
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This product has been used in:
Corti D et al. Analysis of memory B cell responses and isolation of novel monoclonal antibodies with neutralizing breadth from HIV-1-infected individuals. PLoS One 5:e8805 (2010). ELISA; Human. PubMed: 20098712
If you publish research using ab6748 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to IgG
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Rabbit polyclonal Secondary Antibody to Sheep IgG - H&L (AP) - more information
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