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Rabbit anti-Sheep IgG H&L (FITC) secondary antibody (ab6743)

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Overview

Product name

Rabbit anti-Sheep IgG H&L (FITC) secondary antibody
IgG 抗体 (1693件) 一覧

Description

Rabbit polyclonal Secondary Antibody to Sheep IgG - H&L (FITC)

Target species

Sheep

Tested applications

Immunomicroscopy, Flow Cyt, ICC/IF, IHC-P, IHC-Fr, ELISAmore details

Immunogen

Sheep whole molecule

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

0.01M Na phosphate, 0.25M NaCl, pH7.6, 0.05% Na azide, 0.01% Thimerisol, 15mg/ml BSA

Concentration

Concentration information loading...

Purity

IgG fraction

Purification notes

Diaminotriazinylaminofluorescein (DTAF) (Molecular Weight 530 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 2.3 moles DTAF per mole of Rabbit IgG.

Conjugation notes

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Clonality

Polyclonal

Research areas

Secondary antibodies >> anti-Sheep >> IgG >> Fluorophore >> FITC
Immunology >> Immunoglobulins >> Heavy Chain >> IgG

Applications

Show applications key

Our Abpromise guarantee covers the use of ab6743 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Immunomicroscopy

     

  • Flow Cyt

     

  • ICC/IF

     

  • IHC-P

     

  • IHC-Fr

     

  • ELISA

     

Application notes

Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency

References for Rabbit anti-Sheep IgG H&L (FITC) secondary antibody (ab6743)

This product has been referenced in:

See all 4 publications for this product

Publishing research using ab6743? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"