Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 4°C
Antigen retrieval step
Other
Sample
Mouse Tissue sections (Fixed mouse brain)
Specification
Fixed mouse brain
Permeabilization
Yes - 0.5% TritonX 100
Fixative
Paraformaldehyde
Other product details
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C
Secondary antibody
Name
Non-Abcam antibody was used: Cy3 conjugated Goat anti rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy3
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy3
Additional data
Additional Notes
Labeled well when treat only this antiboy but didn't in double lebeling.
I think some interupt accure between two primary Ab.
Thus Plz try sequencial treatment of Ab
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Hyun Jin Kim
Verified customer
投稿 Aug 09 2013