Anti-Serum Response Factor SRF (phospho S103) 抗体 (ab53130)
Key features and details
- Rabbit polyclonal to Serum Response Factor SRF (phospho S103)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Serum Response Factor SRF (phospho S103) antibody
Serum Response Factor SRF 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Serum Response Factor SRF (phospho S103) -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human Serum Response Factor SRF aa 50-150 (phospho S103).
Database link: P11831 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2 -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab53130 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab53130の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/300 - 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 52 kDa).
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IHC-P |
Use at an assay dependent concentration.
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特記事項 |
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WB
1/300 - 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 52 kDa). |
IHC-P
Use at an assay dependent concentration. |
ターゲット情報
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機能
SRF is a transcription factor that binds to the serum response element (SRE), a short sequence of dyad symmetry located 300 bp to the 5' of the site of transcription initiation of some genes (such as FOS). Required for cardiac differentiation and maturation. -
配列類似性
Contains 1 MADS-box domain. -
翻訳後修飾
Phosphorylated by PRKDC. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 6722 Human
- Omim: 600589 Human
- SwissProt: P11831 Human
- Unigene: 520140 Human
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別名
- c fos serum response element binding factor antibody
- c fos serum response element binding transcription factor antibody
- ELK3 antibody
see all
画像
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All lanes : Anti-Serum Response Factor SRF (phospho S103) antibody (ab53130) at 1/300 dilution
Lane 1 : LOVO cell extract treated with serum (20%, 15mins)
Lane 2 : LOVO cell extract treated with serum (20%, 15mins) with immunising peptide
Predicted band size: 52 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted? -
ab53130 at 1/50 dilution staining Serum Response Factor SRF in human lung carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence and presence of the immunising peptide.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab53130 は 5 報の論文で使用されています。
- Russell AP et al. Striated muscle activator of Rho signalling (STARS) is reduced in ageing human skeletal muscle and targeted by miR-628-5p. Acta Physiol (Oxf) 220:263-274 (2017). PubMed: 27739650
- Heller EA et al. Locus-specific epigenetic remodeling controls addiction- and depression-related behaviors. Nat Neurosci 17:1720-7 (2014). PubMed: 25347353
- Vissing K et al. Effect of resistance exercise contraction mode and protein supplementation on members of the STARS signalling pathway. J Physiol 591:3749-63 (2013). PubMed: 23753523
- Kawashima T et al. Synaptic activity-responsive element in the Arc/Arg3.1 promoter essential for synapse-to-nucleus signaling in activated neurons. Proc Natl Acad Sci U S A 106:316-21 (2009). EMSA . PubMed: 19116276
- Xiao Q et al. Embryonic stem cell differentiation into smooth muscle cells is mediated by Nox4-produced H2O2. Am J Physiol Cell Physiol 296:C711-23 (2009). PubMed: 19036941