Application
Western blot
Sample
Human Serum (Serum)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
Serum
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Other product details
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1xTBST with 1% milk
Dilution
1/500
Secondary antibody
Dilution
1/3000
Name
Non-Abcam antibody was used: DAKO
Host species: Rabbit
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 25 kDa kDa
Positive control
Human serum.
Additional data
Additional Notes
Human serum samples run in Bis-Tris 4-12% gradient gel and then transfered in PVDF membrane, probed with rabbit monoclonal (Ab45151) antibody. It showed very clear one 25kDA band. Very clean good antibody.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Ruma Raha-Chowdhury
Verified customer
投稿 Jun 25 2015