Application
Western blot
Sample
Human Cell lysate - whole cell (Bxpc3)
Gel Running Conditions
Reduced Denaturing (4-20% tris-Glycin gel)
Loading amount
100000 cells
Specification
Bxpc3
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk in TBST
Dilution
1/1000
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: #7074S anti-rabbit IgG Cell Signaling
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ez-ecl
Exposure
5 second(s)
Bands
Specific: 46 kDa Non-specific: 74 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Dec 12 2013