Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (hct-116)
Antigen retrieval step
None
Permeabilization
Yes - 0.1% TRITON IN PBS
Specification
hct-116
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 0.5% · Temperature: R.T°C
Fixative
Paraformaldehyde
Other product details
Incubation time
2 hour(s) and 0 minute(s)
Dilution
1/500
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: Donkey anti-Rabbit IgG (H+L) Secondary Antibody, A
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Nov 11 2016