Anti-AANAT 抗体 (ab3505)
Key features and details
- Rabbit polyclonal to AANAT
- Suitable for: IHC-P, WB
- Reacts with: Rat
- Isotype: IgG
製品の概要
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製品名
Anti-AANAT antibody
AANAT 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to AANAT -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Rat
交差が予測される動物種: Mouse, Chicken, Hamster, Human, Chimpanzee, Rhesus monkey -
免疫原
Synthetic peptide corresponding to Mouse AANAT aa 1-100 (N terminal).
Database link: O88816 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Total anti-N-terminal AA-NAT-specific IgG was affinity purified on resin-coupled non-phosphopeptide. Phospho-specific amino-terminal IgG was subsequently isolated on resin-coupled phosphopeptide. -
一次抗体 備考
Pineal gland serotonin N-acetyltransferase (arylalkylamine N-acetyltransferase, AA-NAT) is an ~23 kDa protein which is the rate-limiting enzyme in melatonin synthesis. Melatonin serves as the hormonal signal of the daily light/dark cycle, or body clock. The nocturnal oscillation in enzyme activity ranges by 10- to 100-fold depending on species among vertebrates. Studies have shown that AA-NAT is regulated by cAMP at both the mRNA and protein levels. AA-NAT has putative protein kinase A (PKA) sites at Thr 29 and Ser 206. Phosphorylation of AA-NAT at these residues might play a role in protein inactivation via proteasome-dependent degradation. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab3505の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 23 kDa.
This antibody detects an ~23 kDa protein representing unphosphorylated and phosphorylated AA-NAT from rat pineal gland whole protein extract. |
特記事項 |
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IHC-P
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 23 kDa. This antibody detects an ~23 kDa protein representing unphosphorylated and phosphorylated AA-NAT from rat pineal gland whole protein extract. |
ターゲット情報
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機能
Controls the night/day rhythm of melatonin production in the pineal gland. Catalyzes the N-acetylation of serotonin into N-acetylserotonin, the penultimate step in the synthesis of melatonin. -
組織特異性
Highly expressed in pineal gland and at lower levels in the retina. Weak expression in several brain regions and in the pituitary gland. -
パスウェイ
Aromatic compound metabolism; melatonin biosynthesis; melatonin from serotonin: step 1/2. -
関連疾患
Delayed sleep phase syndrome -
配列類似性
Belongs to the acetyltransferase family. AANAT subfamily.
Contains 1 N-acetyltransferase domain. -
翻訳後修飾
cAMP-dependent phosphorylation on both N-terminal Thr-31 and C-terminal Ser-205 regulates AANAT activity by promoting interaction with 14-3-3 proteins. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 503504 Chimpanzee
- Entrez Gene: 15 Human
- Entrez Gene: 11298 Mouse
- Entrez Gene: 25120 Rat
- Omim: 600950 Human
- SwissProt: P79774 Chicken
- SwissProt: Q5IS55 Chimpanzee
- SwissProt: Q16613 Human
see all -
別名
- AA-NAT antibody
- Aanat antibody
- Aralkylamine N-acetyltransferase antibody
see all
データシートおよび資料
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Datasheet download
参考文献 (12)
ab3505 は 12 報の論文で使用されています。
- Yang C et al. Melatonin delays ovarian aging in mice by slowing down the exhaustion of ovarian reserve. Commun Biol 4:534 (2021). PubMed: 33958705
- Yang M et al. Melatonin promotes male reproductive performance and increases testosterone synthesis in mammalian Leydig cells†. Biol Reprod 104:1322-1336 (2021). PubMed: 33709108
- Wang J et al. Melatonin Alleviates the Suppressive Effect of Hypoxanthine on Oocyte Nuclear Maturation and Restores Meiosis via the Melatonin Receptor 1 (MT1)-Mediated Pathway. Front Cell Dev Biol 9:648148 (2021). PubMed: 33937242
- Song L et al. Roseburia hominis Increases Intestinal Melatonin Level by Activating p-CREB-AANAT Pathway. Nutrients 14:N/A (2021). PubMed: 35010992
- Yi G et al. A study of the mechanism of small-molecule soybean-protein-derived peptide supplement to promote sleep in a mouse model. RSC Adv 10:11264-11273 (2020). PubMed: 35495343