Application
Western blot
Sample
Mouse Cell lysate - whole cell (whole)
Gel Running Conditions
Reduced Denaturing (4-20%)
Loading amount
50 µg
Treatment
500nM NE
Specification
whole
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk in TBS-T
Concentration
0.5 µg/ml
Secondary antibody
Name
Non-Abcam antibody was used: Anti-rabbit IgG, HRP-linked Antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Detection
Detection method
ECL
Exposure
4 minute(s) and 0 second(s)
Bands
Specific: ~67 kDa Non-specific: ~50 kDa
Positive control
Mouse brain lysate
Additional data
Additional Notes
Excellent antibody for Western blot analysis
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Jul 20 2017