Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (spinal cord)
Specification
spinal cord
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citratbuffer pH6
Permeabilization
No
Blocking step
Endogenous peroxidase was inhibited by 1% H2O2in methanol for 15min. Sections were pretreated with 10% normal goat serum as blocking agent for 15 minute(s) · Concentration: 10% · Temperature: 22°C
Other product details
Dilution
1/100
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS with 1% Serum
Secondary antibody
Name
Non-Abcam antibody was used: goat anti-rabbit Immunoglobulins/biotylated DAKO
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/400
Additional data
PubMed ID
58:1748–1756: View
Additional Notes
After 30min secondary antibody the antibody complexes were visualized with peroxidase-conjugated streptavidin-biotin using diaminobenzidine substrate and counterstained with Mayer ’s hemalaun.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MS. Nicole Gatzemeier
Verified customer
投稿 Feb 15 2011