Anti-Semaphorin 7a 抗体 (ab23578)
Key features and details
- Rabbit polyclonal to Semaphorin 7a
- Suitable for: WB, IP
- Reacts with: Mouse, Rat
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
-
製品名
Anti-Semaphorin 7a antibody
Semaphorin 7a 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Semaphorin 7a -
由来種
Rabbit -
特異性
ab23578 detects Semaphorin 7a protein at 75 kDa by WB. Semaphorin 7a is a protein that is tethered to the cell membrane via a GPI-anchor and is also glycosylated, therefore it is not surprising that in WB we see some additional bands at ~50 and 100 kDa which are likely to be cleavage fragments and glycosylated protein respectively. Preincubation with the immunizing peptide ab30844 is successful in blocking antibody staining. -
アプリケーション
適用あり: WB, IPmore details
適用なし: ICC/IF -
種交差性
交差種: Mouse, Rat
交差が予測される動物種: Cow -
免疫原
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Mouse Semaphorin 7a.
-
ポジティブ・コントロール
- WB: Mouse and rat brain tissue lysate. IP: Mouse brain tissue lysate.
-
特記事項
Negative control: HEK cellsThe Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
-
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab23578の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 75, 90 kDa (predicted molecular weight: 75 kDa).
|
|
IP |
Use at an assay dependent concentration.
|
特記事項 |
---|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 75, 90 kDa (predicted molecular weight: 75 kDa). |
IP
Use at an assay dependent concentration. |
ターゲット情報
-
機能
Plays an important role in integrin-mediated signaling and functions both in regulating cell migration and immune responses. Promotes formation of focal adhesion complexes, activation of the protein kinase PTK2/FAK1 and subsequent phosphorylation of MAPK1 and MAPK3. Promotes production of proinflammatory cytokines by monocytes and macrophages. Plays an important role in modulating inflammation and T-cell-mediated immune responses. Promotes axon growth in the embryonic olfactory bulb. Promotes attachment, spreading and dendrite outgrowth in melanocytes. -
組織特異性
Detected in skin keratinocytes and on endothelial cells from skin blood vessels (at protein level). Expressed in fibroblasts, keratinocytes, melanocytes, placenta, testis, ovary, spleen, brain, spinal chord, lung, heart, adrenal gland, lymph nodes, thymus, intestine and kidney. -
配列類似性
Belongs to the semaphorin family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 PSI domain.
Contains 1 Sema domain. -
細胞内局在
Cell membrane. Detected in a punctate pattern on the cell membrane of basal and supra-basal skin keratinocytes. - Information by UniProt
-
参照データベース
- Entrez Gene: 20361 Mouse
- Entrez Gene: 315711 Rat
- SwissProt: Q9QUR8 Mouse
- Unigene: 335187 Mouse
-
別名
- CD 108 antibody
- CD108 antibody
- CD108 antigen antibody
see all
画像
-
All lanes : Anti-Semaphorin 7a antibody (ab23578) at 1 µg/ml
Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse cerebellum tissue lysate
Lane 3 : Rat brain tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
Predicted band size: 75 kDa
Observed band size: 75 kDa
Additional bands at: 90 kDa (possible glycosylated form)
Exposure time: 8 minutesGel type: MOPS
Blocking buffer: 2% BSA block
-
Semaphorin 7a was immunoprecipitated using 0.5mg Mouse Brain whole tissue lysate, 5µg of Rabbit polyclonal to Semaphorin 7a and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Mouse Brain whole tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab23578.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 75kDa: Semaphorin 7a.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (21)
ab23578 は 21 報の論文で使用されています。
- Su Y et al. Astrocyte endfoot formation controls the termination of oligodendrocyte precursor cell perivascular migration during development. Neuron 111:190-201.e8 (2023). PubMed: 36384142
- Vilallongue N et al. Guidance landscapes unveiled by quantitative proteomics to control reinnervation in adult visual system. Nat Commun 13:6040 (2022). PubMed: 36229455
- Liu Y et al. Proteomic Analysis Revealed the Characteristics of Key Proteins Involved in the Regulation of Inflammatory Response, Leukocyte Transendothelial Migration, Phagocytosis, and Immune Process during Early Lung Blast Injury. Oxid Med Cell Longev 2021:8899274 (2021). PubMed: 34007409
- Köhler D et al. Red blood cell-derived semaphorin 7A promotes thrombo-inflammation in myocardial ischemia-reperfusion injury through platelet GPIb. Nat Commun 11:1315 (2020). PubMed: 32161256
- Liu Y et al. LncRNA LOXL1-AS1/miR-28-5p/SEMA7A axis facilitates pancreatic cancer progression. Cell Biochem Funct 38:58-65 (2020). PubMed: 31732974