Application
Immunohistochemistry (Frozen sections)
Sample
Chicken Tissue sections (Chick embryo E11)
Specification
Chick embryo E11
Fixative
Paraformaldehyde
Permeabilization
Yes - 0,2% Triton X-100 in PBS, 10'
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Other product details
Dilution
1/200
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 0,5% BSA, 2,5% serum in PBS
Secondary antibody
Name
Non-Abcam antibody was used: Donkey anti-goat Alexa 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/400
Additional data
Additional Notes
Works very nicely with PAF fixed tissue, has low background. Doesn't work with Zn-formalin fixative. In the photo there are skeletal muscle fibres stained with Sema5a antibody.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mrs. Elena Astanina
Verified customer
投稿 Jun 02 2008