Application
Western blot
Sample
Mouse Cell lysate - whole cell (MPRO (mouse promyelocytic) cells)
Loading amount
50 µg
Specification
MPRO (mouse promyelocytic) cells
Gel Running Conditions
Reduced Denaturing (12% Bis-TRIS in MOPS buffer)
Blocking step
Odyssey Blocking Agent (Lycor system) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Other product details
Dilution
1/500
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk/TBST
Secondary antibody
Name
Non-Abcam antibody was used: Lycor Goat anti Rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Dilution
1/10000
Detection
Detection method
Lycor Scanner
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: none kDa Non-specific: 65 kDa
Additional data
Additional Notes
Legend
Lane 1:HEK293 human cell lysate
Lane 2: MPRO mouse cell lysate
Lane 3: S. Cerevisiae lysate
No band was observed at the expected size of 22 kDa.
Lane 1:HEK293 human cell lysate
Lane 2: MPRO mouse cell lysate
Lane 3: S. Cerevisiae lysate
No band was observed at the expected size of 22 kDa.
Abcam response
Thank you for your honest and valuable feedback. Abcam has not tested ab26273 in mouse cells (MPRO mouse promyelocytic cells) for western blot and your data suggest it may not be suitable for this species or application under the conditions in which you tested. Many thanks for your feedback on this antibody.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Feb 05 2010