Application
Flow Cytometry
Sample
Rat Cell (mammary fat pad cells)
Permeabilization
No
Gating Strategy
live cells were gated on side scatter and ab95439 + anti-rabbit 647 based on anti-rabbit 647 control
Specification
mammary fat pad cells
Preparation
Cell harvesting/tissue preparation method: Followed the dissociation and generation of single-cell suspensions from STEMCELL Technologies'
Sample buffer: HBSS + 2% FBS
Sample buffer: HBSS + 2% FBS
Fixation
not fixed
Other product details
Incubation time
30 minute(s) · Diluent: HBSS + 2% FBS
Secondary antibody
Dilution
1/500
Name
Non-Abcam antibody was used: Anti-Rabbit Alexa Fluor 647
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 May 17 2018