Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse Embryonic Stem Cells)
Loading amount
200000 cells
Specification
Mouse Embryonic Stem Cells
Gel Running Conditions
Reduced Denaturing (10% gel)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
Other product details
Dilution
1/500
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% Milk
Secondary antibody
Name
Non-Abcam antibody was used: Dako Polyclonal Goat anti-Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000
Detection
Detection method
Optiblot ECL Ultra Detect Kit
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: 100 kDa
Positive control
Sall4 is an important factor in maintaining mouse ES cell pluripotency; therefore I would expect it to be present in wt D3 mouse ES cells.
Additional data
Additional Notes
Lane 1: D3 (Mouse embryonic stem cell line) whole cell lysate – Anti-Sall4 antibody (ab29112) at 3 µg/ml.
Lane 2: Ecad-/- ES cells (derived from D3 mouse embryonic stem cell line) whole cell lysate – Anti-Sall4 antibody (ab29112) at 3 µg/ml.
Lane 2: Ecad-/- ES cells (derived from D3 mouse embryonic stem cell line) whole cell lysate – Anti-Sall4 antibody (ab29112) at 3 µg/ml.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Joe Segal
Verified customer
投稿 Oct 26 2012