Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Sample
Rat Tissue sections (Brain)
Specification
Brain
Permeabilization
No
Fixative
Formaldehyde
Other product details
Incubation time
2 hour(s) and 10 minute(s) · Temperature: 21°C · Diluent: TBS/BSA/azide
Dilution
1/5000
Secondary antibody
Name
Non-Abcam antibody was used: anti Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/250
Additional data
Additional Notes
An identical positivity to that found in adult mouse brain.
Submitted composite shows widespread, granular positivity in synaptic regions of the Thalamic nucleus ( upper image) and a similar positivity around the cells of the Hippocampal CA3 region ( lower image).
Positivity appears to localise to synaptic regions throughout the brain.
For example there is intense positivity in the Glomeruli of the Granule cell layer of the cerebellum ( not shown).
Additional images can be seen on the Immunoportal website.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Carl Hobbs
Verified customer
投稿 Jun 06 2013