Application
Western blot
Sample
Mouse Cell lysate - whole cell (fibroblast tumor cell)
Loading amount
10 µg
Specification
fibroblast tumor cell
Gel Running Conditions
Reduced Denaturing (4-15%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C
Other product details
Dilution
1/1000
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk
Secondary antibody
Name
Non-Abcam antibody was used: anti-mouse HRP
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2500
Detection
Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 85 kDa
Positive control
SVB6 cells
Negative control
4T1 cells
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. Manish Garg
Verified customer
投稿 Oct 01 2008