Application
Immunoprecipitation
Immuno-precipitation step
Protein A
Sample
Human Cell lysate - whole cell (SH-5YSY differentiated into neurons)
Specification
SH-5YSY differentiated into neurons
Total protein in input
30 µg
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: non-denaturing lysis buffer
Dilution
1/100
Western Blot antibody
Name
Non-Abcam antibody was used: SV2A NBP1-82964
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/250
Additional data
Additional Notes
Lane 1: 30ug of SH-SY5Y differentiated lysate (input), Lane 2: IgG, Lane 3: IP, 500ug of protein used in IP.
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投稿 Jan 29 2015