Application
Western blot
Sample
Human Cell lysate - whole cell (skin)
Loading amount
10 µg
Specification
skin
Treatment
SiRNA SUN1for 48h
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Dilution
1/500
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5%milk in TBST
Secondary antibody
Name
Non-Abcam antibody was used: anti-rabbit IgG
Host species: Mouse
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Mouse
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000
Detection
Detection method
ECL+
Exposure
30 minute(s) and 0 second(s)
Bands
Specific: 90 kDa Non-specific: 80 kDa
Positive control
SiRNA SUN1
Negative control
SiRNA control
Additional data
Additional Notes
exposure should be longer
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Aug 10 2012