製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to SUGT1
  • 特異性
    ab30931 appears to detect both recorded isoforms of SGT1, at 41 kDa and 38 kDa.
  • アプリケーション
    適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Human SUGT1.

    (Peptide available as ab37532.)

  • ポジティブ・コントロール
    • ab30931 gave a positive result in the following whole cell lysates: HeLa (Human epithelial carcinoma cell line) Jurkat (Human T cell lymphoblast-like cell line) A431 (Human epithelial carcinoma cell line) HEK 293 (Human embryonic kidney cell line)

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab30931 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).

ターゲット情報

  • 機能
    May play a role in ubiquitination and subsequent proteasomal degradation of target proteins.
  • 配列類似性
    Belongs to the SGT1 family.
    Contains 1 CS domain.
    Contains 1 SGS domain.
    Contains 3 TPR repeats.
  • ドメイン
    The CS domain mediates interaction with HSP90.
  • 翻訳後修飾
    Phosphorylated at Ser-281 and Ser-331, dephosphorylation promotes nuclear translocation, most likely due to disruption of the SUGT1-HSP90 complex.
  • 細胞内局在
    Cytoplasm. Nucleus. Translocates to the nucleus upon heat shock, requiring S100A6.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Protein 40 6 3 antibody
    • Protein 40-6-3 antibody
    • Protein SGT1 homolog antibody
    • Putative 40 6 3 protein antibody
    • Sgt1 antibody
    • SGT1 suppressor of G2 allele of SKP1 antibody
    • SGT1_HUMAN antibody
    • SGT1B protein antibody
    • sugt1 antibody
    • Suppressor of G2 allele of SKP1 homolog antibody
    • Suppressor of G2 allele of SKP1 S. cerevisiae homolog of antibody
    see all

画像

  • All lanes : Anti-SUGT1 antibody (ab30931) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat whole cell lysate (ab7899)
    Lane 3 : A431 whole cell lysate (ab7909)
    Lane 4 : HEK293 whole cell lysate (ab7902)

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 41 kDa
    Observed band size : 41 kDa
    Additional bands at : 38 kDa (possible isoform).
  • ICC/IF image of ab30931 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab30931, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

参考文献

This product has been referenced in:
  • Wang N  et al. Comparative proteome profile of immature rat ovary during primordial follicle assembly and development. Proteomics 9:3425-34 (2009). Read more (PubMed: 19557761) »

See 1 Publication for this product

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa Cells)
Specification
HeLa Cells
Fixative
Methanol
Permeabilization
No
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 24°C
Username

Abcam user community

Verified customer

投稿 Feb 23 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293 cells)
Loading amount
30 µg
Specification
HEK293 cells
Gel Running Conditions
Reduced Denaturing (12.5% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Abcam user community

Verified customer

投稿 Feb 23 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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