Anti-STUB1/CHIP 抗体 [EPR4448] (ab109103)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4448] to STUB1/CHIP
- Suitable for: WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-STUB1/CHIP antibody [EPR4448]
STUB1/CHIP 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR4448] to STUB1/CHIP -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details
適用なし: Flow Cyt or IHC-P -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- HeLa, MCF7, ECV304, and 293T cell lysates; HeLa cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR4448 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab109103の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 35 kDa.
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ICC/IF |
1/100 - 1/250.
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特記事項 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 35 kDa. |
ICC/IF
1/100 - 1/250. |
ターゲット情報
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機能
E3 ubiquitin-protein ligase which targets misfolded chaperone substrates towards proteasomal degradation. Ubiquitinates NOS1 in concert with Hsp70 and Hsp40. Modulates the activity of several chaperone complexes, including Hsp70, Hsc70 and Hsp90. Mediates transfer of non-canonical short ubiquitin chains to HSPA8 that have no effect on HSPA8 degradation. Mediates polyubiquitination of DNA polymerase beta (POLB) at 'Lys-41', 'Lys-61' and 'Lys-81', thereby playing a role in base-excision repair: catalyzes polyubiquitination by amplifying the HUWE1/ARF-BP1-dependent monoubiquitination and leading to POLB-degradation by the proteasome. Mediates polyubiquitination of CYP3A4. -
組織特異性
Highly expressed in skeletal muscle, heart, pancreas, brain and placenta. Detected in kidney, liver and lung. -
パスウェイ
Protein modification; protein ubiquitination. -
配列類似性
Contains 3 TPR repeats.
Contains 1 U-box domain. -
ドメイン
The TPR domain is essential for ubiquitination mediated by UBE2D1. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR.
Auto-ubiquitinated; mediated by UBE2D1 and UBE2D2. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 10273 Human
- Entrez Gene: 56424 Mouse
- Entrez Gene: 287155 Rat
- Omim: 607207 Human
- SwissProt: Q9UNE7 Human
- SwissProt: Q9WUD1 Mouse
- Unigene: 592081 Human
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別名
- Antigen NY CO 7 antibody
- Antigen NY-CO-7 antibody
- C terminus of Hsp70-interacting protein antibody
see all
画像
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All lanes : Anti-STUB1/CHIP antibody [EPR4448] (ab109103) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : MCF7 cell lysate
Lane 3 : ECV304 cell lysate
Lane 4 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 35 kDa -
Immunofluorescent staining of STUB1/CHIP in HeLa cells using ab109103 at 1/100 dilution.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab109103 は 4 報の論文で使用されています。
- Johnson OT et al. Two distinct classes of cochaperones compete for the EEVD motif in heat shock protein 70 to tune its chaperone activities. J Biol Chem 298:101697 (2022). PubMed: 35148989
- Sugatani J et al. Threonine-408 Regulates the Stability of Human Pregnane X Receptor through Its Phosphorylation and the CHIP/Chaperone-Autophagy Pathway. Drug Metab Dispos 44:137-50 (2016). PubMed: 26534988
- El-Kasaby A et al. A cytosolic relay of heat shock proteins HSP70-1A and HSP90ß monitors the folding trajectory of the serotonin transporter. J Biol Chem 289:28987-9000 (2014). PubMed: 25202009
- Bergmayr C et al. Recruitment of a cytoplasmic chaperone relay by the A2A adenosine receptor. J Biol Chem 288:28831-44 (2013). PubMed: 23965991