Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (splen)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pressure camera
Permeabilization
No
Specification
splen
Blocking step
No blocking step used for 30 minute(s) · Temperature: 25°C
Fixative
Formaldehyde
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/200
Secondary antibody
Name
Non-Abcam antibody was used: Envision + Dual link System-HRP
Host species: Mouse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Mouse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Additional data
Additional Notes
Before antigen retrieval step: 10 consecutive washes with distilled water
For antigen retriveal step with pressure camera was used Tris Edta pH 9 buffer.
Block of endogenous peroxidase: methanol + hydrogen peroxide solucion (8%).
Between each step was perfomed a washing step with Tris HCl pH 7,4, during 5 minutes each one.
Note:
DISCOUNT CODE: 51297
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jan 29 2018