Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Mammary gland)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer ph 6 - using a pressure cooker
Permeabilization
Yes - Triton100-X
Specification
Mammary gland
Blocking step
MOM Mouse IgG blocking reagent as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2µg/mL · Temperature: 25°C
Fixative
Formaldehyde
Other product details
Incubation time
30 minute(s) · Temperature: 25°C
Dilution
1/200
Secondary antibody
Dilution
1/500
Name
Non-Abcam antibody was used: Donkey anti-Mouse Alexa 594
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor 594
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor 594
Additional data
Additional Notes
The blocking reagent and the secondary antibody is from a Mouse-on mouse kit from another company. They do not disclose the concentration.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Flavia Ghiraldini
Verified customer
投稿 Sep 27 2017