Anti-STAT3 抗体 [9D8] (ab119352)
Key features and details
- Mouse monoclonal [9D8] to STAT3
- Suitable for: Flow Cyt, ICC/IF, WB, IP, IHC-P
- Reacts with: Mouse, Rat, Human, African green monkey
- Isotype: IgG
製品の概要
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製品名
Anti-STAT3 antibody [9D8]
STAT3 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [9D8] to STAT3 -
由来種
Mouse -
特異性
Detects STAT3 from Human, murine, monkey and rat samples. -
アプリケーション
適用あり: Flow Cyt, ICC/IF, WB, IP, IHC-Pmore details -
種交差性
交差種: Mouse, Rat, Human, African green monkey -
免疫原
Recombinant fragment, corresponding to amino acids 665-770 of Human STAT3.
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ポジティブ・コントロール
- HepG2, HeLa, PC-12, A549, 293T, Jurkat, A431, U2OS, MCF7, A549, K562, NIH3T3, C2C12, and NRK whole cell lysates; U2OS lysate from STAT3 SMART pool siRNA transfected; Mouse lung tissue lysate; HeLa cells; Human pancreas, brain tumour, colon cancer and stomach cancer tissues Flow Cyt: HeLa cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.1% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
9D8 -
アイソタイプ
IgG -
研究分野
関連製品
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Assay kits
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab119352の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt |
Use 0.1µg for 106 cells.
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ICC/IF |
1/100.
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WB | (2) |
1/5000. Detects a band of approximately 88 kDa (predicted molecular weight: 88 kDa).
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IP |
Use at 2 µg/mg of lysate.
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IHC-P | (5) |
1/1600. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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Flow Cyt
Use 0.1µg for 106 cells. |
ICC/IF
1/100. |
WB
1/5000. Detects a band of approximately 88 kDa (predicted molecular weight: 88 kDa). |
IP
Use at 2 µg/mg of lysate. |
IHC-P
1/1600. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Signal transducer and transcription activator that mediates cellular responses to interleukins, KITLG/SCF, LEP and other growth factors. Once activated, recruits coactivators, such as NCOA1 or MED1, to the promoter region of the target gene (PubMed:17344214). May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the interleukin-6 (IL-6)-responsive elements identified in the promoters of various acute-phase protein genes. Activated by IL31 through IL31RA. Involved in cell cycle regulation by inducing the expression of key genes for the progression from G1 to S phase, such as CCND1 (PubMed:17344214). Mediates the effects of LEP on melanocortin production, body energy homeostasis and lactation (By similarity). May play an apoptotic role by transctivating BIRC5 expression under LEP activation (PubMed:18242580). Cytoplasmic STAT3 represses macroautophagy by inhibiting EIF2AK2/PKR activity. -
組織特異性
Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. -
関連疾患
Hyperimmunoglobulin E recurrent infection syndrome, autosomal dominant
Autoimmune disease, multisystem, infantile-onset -
配列類似性
Belongs to the transcription factor STAT family.
Contains 1 SH2 domain. -
翻訳後修飾
Tyrosine phosphorylated upon stimulation with EGF. Tyrosine phosphorylated in response to constitutively activated FGFR1, FGFR2, FGFR3 and FGFR4 (By similarity). Activated through tyrosine phosphorylation by BMX. Tyrosine phosphorylated in response to IL6, IL11, LIF, CNTF, KITLG/SCF, CSF1, EGF, PDGF, IFN-alpha, LEP and OSM. Activated KIT promotes phosphorylation on tyrosine residues and subsequent translocation to the nucleus. Phosphorylated on serine upon DNA damage, probably by ATM or ATR. Serine phosphorylation is important for the formation of stable DNA-binding STAT3 homodimers and maximal transcriptional activity. ARL2BP may participate in keeping the phosphorylated state of STAT3 within the nucleus. Upon LPS challenge, phosphorylated within the nucleus by IRAK1. Upon erythropoietin treatment, phosphorylated on Ser-727 by RPS6KA5. Phosphorylation at Tyr-705 by PTK6 or FER leads to an increase of its transcriptional activity. Dephosphorylation on tyrosine residues by PTPN2 negatively regulates IL6/interleukin-6 signaling. -
細胞内局在
Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm. Translocated into the nucleus upon tyrosine phosphorylation and dimerization, in response to signaling by activated FGFR1, FGFR2, FGFR3 or FGFR4. Constitutive nuclear presence is independent of tyrosine phosphorylation. Predominantly present in the cytoplasm without stimuli. Upon leukemia inhibitory factor (LIF) stimulation, accumulates in the nucleus. The complex composed of BART and ARL2 plays an important role in the nuclear translocation and retention of STAT3. Identified in a complex with LYN and PAG1. - Information by UniProt
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参照データベース
- Entrez Gene: 6774 Human
- Entrez Gene: 20848 Mouse
- Entrez Gene: 25125 Rat
- Omim: 102582 Human
- SwissProt: P40763 Human
- SwissProt: P42227 Mouse
- SwissProt: P52631 Rat
- Unigene: 463059 Human
see all -
別名
- 1110034C02Rik antibody
- Acute Phase Response Factor antibody
- Acute-phase response factor antibody
see all
画像
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All lanes : Anti-STAT3 antibody [9D8] (ab119352) at 1/1000 dilution
Lane 1 : U2OS lysate from non-targeting control
Lane 2 : U2OS lysate from STAT3 SMART pool siRNA transfected
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : goat anti-mouse-HRP at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 88 kDa
4-20% Tris-HCl polyacrylamide gel -
Overlay histogram showing HeLa cells stained with ab119352 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween 20 for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab119352, 0.1μg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG (ab37355, 0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
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All lanes : Anti-STAT3 antibody [9D8] (ab119352) at 1/5000 dilution
Lane 1 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2 : A-431 (Human epidermoid carcinoma cell line) whole cell lysate
Lane 3 : Hep G2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 4 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 5 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lane 6 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lane 7 : A549 (Human lung carcinoma cell line) whole cell lysate
Lane 8 : Mouse lung tissue lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 88 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted? -
All lanes : Anti-STAT3 antibody [9D8] (ab119352) at 1/5000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : 293T cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : A431 cell lysate
Lane 5 : U2OS cell lysate
Lane 6 : MCF7 cell lysate
Lane 7 : A549 cell lysate
Lane 8 : K562 cell lysate
Lane 9 : COS7 cell lysate
Lane 10 : NIH3T3 cell lysate
Lane 11 : C2C12 cell lysate
Lane 12 : NRK cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : goat anti-mouse-HRP at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 88 kDa
4-20% Tris-HCl polyacrylamide gel -
ab119352, at 1/1600 dilution, staining STAT3 in Human pancreas tissue by Immunohistochemistry. Detection was performed using a goat anti-mouse HRP secondary antibody followed by colorimetric detection using DAB substrate. Tissues were counterstained with hematoxylin.
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Anti-STAT3 antibody [9D8] (ab119352) at 1/1000 dilution + HepG2 total lysate at 25 µg
Secondary
goat anti-mouse-HRP at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 88 kDa
4-20% Tris-HCl polyacrylamide gel -
ab119352, at 1/1600 dilution, staining STAT3 in Human brain tumor tissue by Immunohistochemistry. Detection was performed using a goat anti-mouse HRP secondary antibody followed by colorimetric detection using DAB substrate. Tissues were counterstained with hematoxylin.
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Immunoprecipitation of STAT3 was performed on HepG2 cells. The antigen:antibody complex was formed by incubating 750µg whole cell lysate with 2µg of ab119352 overnight at 4°C. The immune-complex was captured, washed extensively and proteins eluted with 5X Reducing Sample Loading Dye. Samples were resolved on a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to PVDF membrane and blocked with 5% Milk/TBS-0.1% Tween for at least 1 hour. Membranes were then probed with ab119352 at a dilution of 1/5000 overnight at 4°C. Membranes were washed in TBST and probed with IP detection reagent at a dilution of 1/2000 for at least one hour. Membranes were washed and chemiluminescent detection was performed.
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ab119352, at 1/1600 dilution, staining STAT3 in Human colon cancer tissue by Immunohistochemistry. Detection was performed using a goat anti-mouse HRP secondary antibody followed by colorimetric detection using DAB substrate. Tissues were counterstained with hematoxylin.
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ab119352, at 1/1600 dilution, staining STAT3 in Human stomach cancer tissue by Immunohistochemistry. Detection was performed using a goat anti-mouse HRP secondary antibody followed by colorimetric detection using DAB substrate. Tissues were counterstained with hematoxylin.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (181)
ab119352 は 181 報の論文で使用されています。
- Zheng X et al. Circ_0005320 promotes oral squamous cell carcinoma tumorigenesis by sponging microRNA-486-3p and microRNA-637. Bioengineered 13:440-454 (2022). PubMed: 34967281
- Zhang Z et al. Rifaximin protects SH-SY5Y neuronal cells from iron overload-induced cytotoxicity via inhibiting STAT3/NF-κB signaling. Cell Biol Int 46:1062-1073 (2022). PubMed: 35143099
- Xia J et al. Versatile ginsenoside Rg3 liposomes inhibit tumor metastasis by capturing circulating tumor cells and destroying metastatic niches. Sci Adv 8:eabj1262 (2022). PubMed: 35148178
- Wang Y et al. Curcumol Attenuates Endometriosis by Inhibiting the JAK2/STAT3 Signaling Pathway. Med Sci Monit 28:e934914 (2022). PubMed: 35279667
- Chai Y et al. RING-Finger Protein 6 promotes Drug Resistance in Retinoblastoma via JAK2/STAT3 Signaling Pathway. Pathol Oncol Res 28:1610273 (2022). PubMed: 35369571