Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cultured Cells (Ovarian, Breast)
Permeabilization
No
Specification
Ovarian, Breast
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2%
Fixative
Formaldehyde
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/100
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: Anti-mouse-HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Additional data
Additional Notes
Protocol used by customer
-EDTA-Trypsin and then fix them with 10% formaldehyde for 15 min
-Wash and mixed with histogel and then make paraffin block.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Shunyou Wang
Verified customer
投稿 Mar 31 2010