Application
Western blot
Sample
Human Cell lysate - whole cell (HCT116)
Gel Running Conditions
Reduced Denaturing
Loading amount
10 µg
Specification
HCT116
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/2000
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: Goat anti-Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 90 kDa Non-specific: 300, 280, 130, 60 kDa
Positive control
STAT1 alpha antibody ab92506.
Additional data
Additional Notes
In my experimental setting, I cannot tell whether or not this is a phospho-specific S727 antibody (I don' t have negative controls).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Chiara Vardabasso
Verified customer
投稿 Oct 27 2015