Application
Western blot
Sample
Human Cell lysate - whole cell (293-T cells)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
20 µg
Specification
293-T cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/1000
Secondary antibody
Dilution
1/3000
Name
Non-Abcam antibody was used: anti-mouse IgG HRP
Host species: Sheep
Clonality: Polyclonal
Conjugation: HRP polymer
Host species: Sheep
Clonality: Polyclonal
Conjugation: HRP polymer
Detection
Detection method
ECL
Negative control
untransfected, siRNA control
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 50 kDa
Positive control
transfected STAMBPL1, siRNA to STAMBPL1
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
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投稿 Dec 10 2010