Anti-STAM1 抗体 (ab117813)
Key features and details
- Rabbit polyclonal to STAM1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-STAM1 antibody
STAM1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to STAM1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Rabbit, Cow, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan, Elephant -
免疫原
Synthetic peptide, corresponding to a region within amino acids 300-350 of Human STAM1 according to NP_003464.1.
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ポジティブ・コントロール
- Whole cell lysate from HeLa, 293T and Jurkat cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris buffered saline, 0.1% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab117813の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 58 kDa.
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IP |
Use at 2-10 µg/mg of lysate.
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特記事項 |
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WB
1/2000 - 1/10000. Predicted molecular weight: 58 kDa. |
IP
Use at 2-10 µg/mg of lysate. |
ターゲット情報
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機能
Involved in intracellular signal transduction mediated by cytokines and growth factors. Upon IL-2 and GM-CSL stimulation, it plays a role in signaling leading to DNA synthesis and MYC induction. May also play a role in T-cell development. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with HGS (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes. -
組織特異性
Ubiquitously expressed. -
配列類似性
Belongs to the STAM family.
Contains 1 ITAM domain.
Contains 1 SH3 domain.
Contains 1 UIM (ubiquitin-interacting motif) repeat.
Contains 1 VHS domain. -
翻訳後修飾
Phosphorylated on Tyr-198. Phosphorylated in response to IL2, IL3, IL4, IL7, CSF2/GM-CSF, EGF and PDGFB. Phosphorylated by activated PDGFRB. -
細胞内局在
Cytoplasm. Early endosome membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 512734 Cow
- Entrez Gene: 8027 Human
- Omim: 601899 Human
- SwissProt: Q92783 Human
- Unigene: 335391 Human
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別名
- DKFZp686J2352 antibody
- HSE1 homolog antibody
- OTTHUMP00000019237 antibody
see all
画像
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All lanes : Anti-STAM1 antibody (ab117813) at 0.04 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg
Lane 2 : Whole cell lysate from HeLa cells at 15 µg
Lane 3 : Whole cell lysate from 293T cells at 50 µg
Lane 4 : Whole cell lysate from Jurkat cells at 50 µg
Developed using the ECL technique.
Predicted band size: 58 kDa
Exposure time: 30 seconds -
ab117813 at 1 µg/ml staining STAM1 by WB, following immunoprecipitation of whole cell lysate from HeLa cells.
Lane 1; IP using ab117813 at 6 µg/mg lysate.
Lane 1; IP using an antibody to a downstream epitope.
Lane 2; IP using an antibody to an alternative downstream epitope.
Lane 3; IP using control IgG.
1 mg of lysate was used for IP and 20% of IP was loaded. Detection utilised ECL with a 10 second exposure.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (0)
ab117813 は論文での使用が確認できていません。