Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Xenopus laevis Cell lysate - whole cell (Egg)
Specification
Egg
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 23°C
Other product details
Concentration
1 µg/ml
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 23°C
Secondary antibody
Name
Non-Abcam antibody was used: li-cor IRDye 800CW
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye 800CW
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye 800CW
Dilution
1/15000
Detection
Detection method
Odyssey Imaging
Exposure
30 minute(s) and 0 second(s)
Positive control
HeLa cell lysate
Additional data
Abcam response
Currently, we have not tested this antibody in Xenopus laevis samples and therefore cannot guarantee its performance. It would appear that under these experimental conditions, the antibody may not be suitable for use in Xenopus samples. We encourage our researchers to test new species or applications using our AbTrial program.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
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投稿 Feb 18 2014