Application
Western blot
Loading amount
0.25 µg
Gel Running Conditions
Non-reduced Denaturing (12% SDS-PAGE)
Sample
Human Recombinant protein (Baculovirus expression)
Specification
Baculovirus expression
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 18°C · Diluent: PBS tween 5% milk
Dilution
1/2500
Secondary antibody
Name
Non-Abcam antibody was used: Anti mouse
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2500
Detection
Detection method
ECL prime
Bands
Specific: 80 kDa
Exposure
1 minute(s) and 0 second(s)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Aug 30 2013