Anti-SSB 抗体 [EPR6570] (ab124932)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6570] to SSB
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
-
製品名
Anti-SSB antibody [EPR6570]
SSB 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR6570] to SSB -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details
適用なし: Flow Cyt -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human SSB aa 100-200. The exact sequence is proprietary.
-
ポジティブ・コントロール
- Jurkat, K562, HeLa, Ramos, C6, PC12, NIH 3T3 and RAW 264.7 cell lysates; Human colon tissue; Human thyroid adenocarcinoma tissue
-
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解離定数(KD 値)
KD = 4.80 x 10 -11 M Learn more about KD -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
-
精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EPR6570 -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab124932の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
1/10000 - 1/50000. Detects a band of approximately 47 kDa (predicted molecular weight: 46 kDa).
|
|
IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
特記事項 |
---|
WB
1/10000 - 1/50000. Detects a band of approximately 47 kDa (predicted molecular weight: 46 kDa). |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
-
機能
Binds to the 3' poly(U) terminii of nascent RNA polymerase III transcripts, protecting them from exonuclease digestion and facilitating their folding and maturation. -
配列類似性
Contains 1 HTH La-type RNA-binding domain.
Contains 1 RRM (RNA recognition motif) domain. -
翻訳後修飾
Phosphorylated. The phosphorylation sites are at the C-terminal part of the protein.
The N-terminus is blocked. -
細胞内局在
Nucleus. - Information by UniProt
-
参照データベース
- Entrez Gene: 6741 Human
- Entrez Gene: 20823 Mouse
- Entrez Gene: 81783 Rat
- GenBank: NP_003133.1 Human
- Omim: 109090 Human
- SwissProt: P05455 Human
- SwissProt: P32067 Mouse
- SwissProt: P38656 Rat
see all -
別名
- Autoantigen La antibody
- La antibody
- La autoantigen antibody
see all
画像
-
All lanes : Anti-SSB antibody [EPR6570] (ab124932) at 1/10000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : K562 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : Ramos cell lysate
Lane 5 : C6 cell lysate
Lane 6 : PC12 cell lysate
Lane 7 : NIH 3T3 cell lysate
Lane 8 : RAW 264.7 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled Goat anti Rabbit at 1/2000 dilution
Predicted band size: 46 kDa
Observed band size: 47 kDa why is the actual band size different from the predicted? -
Formalin-fixed, paraffin-embedded human colon tissue stained for SSB with ab124932 (1/250 dilution) in immunohistochemical analysis.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
Formalin-fixed, paraffin-embedded human thyroid adenocarcinoma tissue stained for SSB with ab124932 (1/250 dilution) in immunohistochemical analysis.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (1)
ab124932 は 1 報の論文で使用されています。
- Huang X et al. La protein regulates protein expression by binding with the mRNAs of target genes and participates the pathological process of ovarian cancer. Front Oncol 12:763480 (2022). PubMed: 36110943