Application
Western blot
Sample
Mouse Cell lysate - whole cell (HEK293H, COS7, mouse hindbrain lysate E14)
Loading amount
30 µg
Specification
HEK293H, COS7, mouse hindbrain lysate E14
Gel Running Conditions
Reduced Denaturing (4-15% SDS gradient gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/2000
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% Milk
Secondary antibody
Name
Non-Abcam antibody was used: goat anti rabbit IgG-HRP coupled
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/20000
Detection
Detection method
ECL+
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 150 (GFP tagged 170) kDa
Positive control
HEK293/HeLa cells overexpressing human or mouse srGAP1,2,3, untransfected COS7 (specificity of detection doubtful), embryonic mouse hindbrain full lysate (E14)
Additional data
Additional Notes
No cross reactivity with mouse srGAP2 in the overexpressing condition, seems to detect endogenous srGAP- very nice.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Alain Chedotal
Verified customer
投稿 Apr 05 2013