Anti-SREBP2 抗体 (ab30682)
Key features and details
- Rabbit polyclonal to SREBP2
- Suitable for: ICC/IF, WB, IHC-Fr
- Reacts with: Rat, Chicken, Human
- Isotype: IgG
製品の概要
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製品名
Anti-SREBP2 antibody
SREBP2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to SREBP2 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WB, IHC-Frmore details -
種交差性
交差種: Rat, Chicken, Human
交差が予測される動物種: Guinea pig, Pig, Xenopus laevis, Chinese hamster -
免疫原
Synthetic peptide corresponding to Human SREBP2 aa 455-469.
Sequence:SPLLDDAKVKDEPDS
Database link: Q12772 -
ポジティブ・コントロール
- ICC: HeLa cells. WB: Human fibroblast cell lysate, Rat testis supernatant, Human retina whole cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 50% Glycerol, 0.1% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
- Epigenetics and Nuclear Signaling
- Transcription
- Domain Families
- HLH / Leucine Zipper
- HLH / Leucine Zipper
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
関連製品
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Assay kits
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab30682の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
Use a concentration of 1 µg/ml.
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WB | (10) |
1/200 - 1/1000. Detects a band of approximately 126 kDa (predicted molecular weight: 126 kDa).
Block with 5% non-fat dry milk in TBS pH 7.4 for 1-2 hours at room temperature or over night at 4°C. |
IHC-Fr |
Use at an assay dependent concentration.
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特記事項 |
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ICC/IF
Use a concentration of 1 µg/ml. |
WB
1/200 - 1/1000. Detects a band of approximately 126 kDa (predicted molecular weight: 126 kDa). Block with 5% non-fat dry milk in TBS pH 7.4 for 1-2 hours at room temperature or over night at 4°C. |
IHC-Fr
Use at an assay dependent concentration. |
ターゲット情報
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機能
Transcriptional activator required for lipid homeostasis. Regulates transcription of the LDL receptor gene as well as the cholesterol and to a lesser degree the fatty acid synthesis pathway (By similarity). Binds the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3') found in the flanking region of the LDRL and HMG-CoA synthase genes. -
組織特異性
Ubiquitously expressed in adult and fetal tissues. -
配列類似性
Belongs to the SREBP family.
Contains 1 basic helix-loop-helix (bHLH) domain. -
翻訳後修飾
At low cholesterol the SCAP/SREBP complex is recruited into COPII vesicles for export from the ER. In the Golgi complex SREBPs are cleaved sequentially by site-1 and site-2 protease. The first cleavage by site-1 protease occurs within the luminal loop, the second cleavage by site-2 protease occurs within the first transmembrane domain and releases the transcription factor from the Golgi membrane. Apoptosis triggers cleavage by the cysteine proteases caspase-3 and caspase-7. -
細胞内局在
Nucleus and Endoplasmic reticulum membrane. Golgi apparatus membrane. Cytoplasmic vesicle > COPII-coated vesicle membrane. Moves from the endoplasmic reticulum to the Golgi in the absence of sterols. - Information by UniProt
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参照データベース
- Entrez Gene: 395304 Chicken
- Entrez Gene: 100689017 Chinese hamster
- Entrez Gene: 6721 Human
- Entrez Gene: 396675 Pig
- Entrez Gene: 300095 Rat
- Entrez Gene: 443980 Xenopus laevis
- Omim: 600481 Human
- SwissProt: Q60429 Chinese hamster
see all -
別名
- AI608257 antibody
- bHLHd2 antibody
- Class D basic helix-loop-helix protein 2 antibody
see all
画像
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Anti-SREBP2 antibody (ab30682) at 1/1000 dilution + Human retina whole cell lysate at 30 µg
Secondary
HRP-conjugated goat anti-rabbit polyclonal at 1/1000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 126 kDa
Additional bands at: 126, 55 kDa, 60 kDa (possible non-specific binding). We are unsure as to the identity of these extra bands.
Exposure time: 6 minutesBlocking: 7% milk for 60 minutes at 20oC.
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ICC/IF image of ab30682 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab30682, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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All lanes : Anti-SREBP2 antibody (ab30682) at 1/200 dilution
Lane 1 : Human fibroblast cell lysate
Lane 2 : Rat brown fat homogenate
Lane 3 : Rat testis supernatant
Lysates/proteins at 60 µg per lane.
Predicted band size: 126 kDa
Observed bands 126 kDa, 55kDa (cleaved form)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (137)
ab30682 は 137 報の論文で使用されています。
- Hu X et al. Inhibition of Stearoyl-CoA Desaturase 1 Potentiates Anti-tumor Activity of Amodiaquine in Non-small Cell Lung Cancer. Biol Pharm Bull 45:438-445 (2022). PubMed: 35110426
- Vidimce J et al. Sexual dimorphism: increased sterol excretion leads to hypocholesterolaemia in female hyperbilirubinaemic Gunn rats. J Physiol 600:1889-1911 (2022). PubMed: 35156712
- Yano H et al. Blocking cholesterol efflux mechanism is a potential target for antilymphoma therapy. Cancer Sci 113:2129-2143 (2022). PubMed: 35343027
- Dai T et al. Black TiO2 nanoprobe-mediated mild phototherapy reduces intracellular lipid levels in atherosclerotic foam cells via cholesterol regulation pathways instead of apoptosis. Bioact Mater 17:18-28 (2022). PubMed: 35386468
- Shibuya K et al. AAA-ATPase valosin-containing protein binds the transcription factor SREBP1 and promotes its proteolytic activation by rhomboid protease RHBDL4. J Biol Chem 298:101936 (2022). PubMed: 35430252