Anti-SREBP2 抗体 (ab112046)
Key features and details
- Rabbit polyclonal to SREBP2
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-SREBP2 antibody
SREBP2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to SREBP2 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Rat, Horse, Chicken, Guinea pig, Cow, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Chinese hamster, Orangutan -
免疫原
Synthetic peptide corresponding to Human SREBP2 aa 300-350.
Database link: Q12772 -
ポジティブ・コントロール
- HeLa and 293T whole cell lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.00
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
- Epigenetics and Nuclear Signaling
- Transcription
- Domain Families
- HLH / Leucine Zipper
- HLH / Leucine Zipper
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
関連製品
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Assay kits
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab112046の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (2) |
1/1000 - 1/5000. Predicted molecular weight: 124 kDa.
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IP |
Use at 2-10 µg/mg of lysate.
|
特記事項 |
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WB
1/1000 - 1/5000. Predicted molecular weight: 124 kDa. |
IP
Use at 2-10 µg/mg of lysate. |
ターゲット情報
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機能
Transcriptional activator required for lipid homeostasis. Regulates transcription of the LDL receptor gene as well as the cholesterol and to a lesser degree the fatty acid synthesis pathway (By similarity). Binds the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3') found in the flanking region of the LDRL and HMG-CoA synthase genes. -
組織特異性
Ubiquitously expressed in adult and fetal tissues. -
配列類似性
Belongs to the SREBP family.
Contains 1 basic helix-loop-helix (bHLH) domain. -
翻訳後修飾
At low cholesterol the SCAP/SREBP complex is recruited into COPII vesicles for export from the ER. In the Golgi complex SREBPs are cleaved sequentially by site-1 and site-2 protease. The first cleavage by site-1 protease occurs within the luminal loop, the second cleavage by site-2 protease occurs within the first transmembrane domain and releases the transcription factor from the Golgi membrane. Apoptosis triggers cleavage by the cysteine proteases caspase-3 and caspase-7. -
細胞内局在
Nucleus and Endoplasmic reticulum membrane. Golgi apparatus membrane. Cytoplasmic vesicle > COPII-coated vesicle membrane. Moves from the endoplasmic reticulum to the Golgi in the absence of sterols. - Information by UniProt
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参照データベース
- Entrez Gene: 395304 Chicken
- Entrez Gene: 100689017 Chinese hamster
- Entrez Gene: 507102 Cow
- Entrez Gene: 6721 Human
- Entrez Gene: 396675 Pig
- Entrez Gene: 300095 Rat
- Omim: 600481 Human
- SwissProt: Q60429 Chinese hamster
see all -
別名
- AI608257 antibody
- bHLHd2 antibody
- Class D basic helix-loop-helix protein 2 antibody
see all
画像
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All lanes : Anti-SREBP2 antibody (ab112046) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 124 kDa
Exposure time: 3 minutes -
Detection of SREBP2 by Western Blot of Immunprecipitate.
Lane 1: ab112046 at 1µg/ml staining SREBP2 in HeLa whole cell lysate immunoprecipitated using ab112046 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Lane 2: Control IgG
Detection: Chemiluminescence with exposure time of 30 seconds.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab112046 は 5 報の論文で使用されています。
- Zhang D et al. TFCP2 Overcomes Senescence by Cooperating With SREBP2 to Activate Cholesterol Synthesis in Pancreatic Cancer. Front Oncol 11:724437 (2021). PubMed: 34804919
- Fan Z et al. Brahma Related Gene 1 (Brg1) Regulates Cellular Cholesterol Synthesis by Acting as a Co-factor for SREBP2. Front Cell Dev Biol 8:259 (2020). PubMed: 32500071
- Hu Y et al. Regulation effects of total flavonoids in Morus alba L. on hepatic cholesterol disorders in orotic acid induced NAFLD rats. BMC Complement Med Ther 20:257 (2020). PubMed: 32807146
- Li X et al. SREBP-2 promotes stem cell-like properties and metastasis by transcriptional activation of c-Myc in prostate cancer. Oncotarget 7:12869-84 (2016). WB . PubMed: 26883200
- Li X et al. Fatostatin displays high antitumor activity in prostate cancer by blocking SREBP-regulated metabolic pathways and androgen receptor signaling. Mol Cancer Ther 13:855-66 (2014). PubMed: 24493696