Anti-SRD5A2 抗体 (ab27469)
Key features and details
- Goat polyclonal to SRD5A2
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-SRD5A2 antibody
SRD5A2 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to SRD5A2 -
由来種
Goat -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide:
QVQCQQSPVLAG
, corresponding to N terminal amino acids 2-13 of Human SRD5A2 -
ポジティブ・コントロール
- Human testes or prostate lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab27469の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 28 kDa).
1 hour primary incubation is recommended for this product. |
特記事項 |
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WB
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 28 kDa). 1 hour primary incubation is recommended for this product. |
ターゲット情報
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機能
Converts testosterone (T) into 5-alpha-dihydrotestosterone (DHT) and progesterone or corticosterone into their corresponding 5-alpha-3-oxosteroids. It plays a central role in sexual differentiation and androgen physiology. -
組織特異性
Expressed in high levels in the prostate and many other androgen-sensitive tissues. -
関連疾患
Defects in SRD5A2 are the cause of pseudovaginal perineoscrotal hypospadias (PPSH) [MIM:264600]. A form of male pseudohermaphroditism in which 46,XY males show ambiguous genitalia at birth, including perineal hypospadias and a blind perineal pouch, and develop masculinization at puberty. The name of the disorder stems from the finding of a blind-ending perineal opening resembling a vagina and a severely hypospadiac penis with the urethra opening onto the perineum. -
配列類似性
Belongs to the steroid 5-alpha reductase family. -
細胞内局在
Microsome membrane. Endoplasmic reticulum membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 6716 Human
- Omim: 607306 Human
- SwissProt: P31213 Human
- Unigene: 458345 Human
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別名
- 3 oxo 5 alpha steroid 4 dehydrogenase 2 antibody
- 3-oxo-5-alpha-steroid 4-dehydrogenase 2 antibody
- 5 alpha SR2 antibody
see all
画像
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Anti-SRD5A2 antibody (ab27469) at 0.3 µg/ml + Human testes lysate (35ug protein in RIPA buffer)
Predicted band size: 28 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour. Detected by chemiluminescence.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab27469 は 5 報の論文で使用されています。
- Samuel RM et al. Androgen Signaling Regulates SARS-CoV-2 Receptor Levels and Is Associated with Severe COVID-19 Symptoms in Men. Cell Stem Cell 27:876-889.e12 (2020). PubMed: 33232663
- Bennett GA et al. Prenatal Stress Alters Hippocampal Neuroglia and Increases Anxiety in Childhood. Dev Neurosci 37:533-45 (2015). PubMed: 26279160
- Kelleher MA et al. Changes in neuroactive steroid concentrations after preterm delivery in the Guinea pig. Reprod Sci 20:1365-75 (2013). PubMed: 23585339
- Kelleher MA et al. Sex-dependent effect of a low neurosteroid environment and intrauterine growth restriction on foetal guinea pig brain development. J Endocrinol 208:301-9 (2011). PubMed: 21149437
- Vu TT et al. Changes in human placental 5alpha-reductase isoenzyme expression with advancing gestation: effects of fetal sex and glucocorticoid exposure. Reprod Fertil Dev 21:599-607 (2009). WB ; Human . PubMed: 19383266