Anti-SRB4 抗体 (ab63812)
Key features and details
- Rabbit polyclonal to SRB4
- Suitable for: WB
- Reacts with: Saccharomyces cerevisiae
- Isotype: IgG
製品の概要
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製品名
Anti-SRB4 antibody -
製品の詳細
Rabbit polyclonal to SRB4 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Saccharomyces cerevisiae -
免疫原
Recombinant full length protein (Saccharomyces cerevisiae), purified by chromatography.
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ポジティブ・コントロール
- Saccharomyces cerevisiae extract.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 6
Preservative: 0.1% Sodium azide
Constituent: Whole serum -
Concentration information loading...
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精製度
Whole antiserum -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab63812の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/5000. Detects a band of approximately 100 kDa (predicted molecular weight: 78 kDa).
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特記事項 |
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WB
1/5000. Detects a band of approximately 100 kDa (predicted molecular weight: 78 kDa). |
ターゲット情報
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機能
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator complex, having a compact conformation in its free form, is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. The Mediator complex unfolds to an extended conformation and partially surrounds RNA polymerase II, specifically interacting with the unphosphorylated form of the C-terminal domain (CTD) of RNA polymerase II. The Mediator complex dissociates from the RNA polymerase II holoenzyme and stays at the promoter when transcriptional elongation begins. -
配列類似性
Belongs to the Mediator complex subunit 17 family. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 856743 Saccharomyces cerevisiae
- SwissProt: P32569 Saccharomyces cerevisiae
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別名
- MED17 antibody
- MED17_YEAST antibody
- Mediator complex subunit 17 antibody
see all
画像
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All lanes : Anti-SRB4 antibody (ab63812) at 1/5000 dilution
Lane 1 : Molecular weight markers
Lane 2 : Saccharomyces cerevisiae extract
Predicted band size: 78 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab63812 は 3 報の論文で使用されています。
- Young CP et al. Distinct histone methylation and transcription profiles are established during the development of cellular quiescence in yeast. BMC Genomics 18:107 (2017). WB ; Saccharomyces cerevisiae . PubMed: 28122508
- Paul E et al. Genome-wide association of mediator and RNA polymerase II in wild-type and mediator mutant yeast. Mol Cell Biol 35:331-42 (2015). ChIP ; Saccharomyces cerevisiae . PubMed: 25368384
- Jeronimo C & Robert F Kin28 regulates the transient association of Mediator with core promoters. Nat Struct Mol Biol 21:449-55 (2014). IP . PubMed: 24704787