Application
Western blot
Sample
Mouse Tissue lysate - whole (Heart)
Loading amount
40 µg
Specification
Heart
Gel Running Conditions
Reduced Denaturing (10)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 30°C
Other product details
Dilution
1/3000
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: BSA
Secondary antibody
Name
Non-Abcam antibody was used: Vector
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
ECL
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 62 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. Mtuhtu S.Gounder
Verified customer
投稿 Dec 02 2011