Anti-SQSTM1 / p62 抗体 (ab155686)
Key features and details
- Rabbit polyclonal to SQSTM1 / p62
- Suitable for: IHC-P, Flow Cyt, IP, WB, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-SQSTM1 / p62 antibody
SQSTM1 / p62 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to SQSTM1 / p62 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, Flow Cyt, IP, WB, ICC/IFmore details -
種交差性
交差種: Rat, Human -
免疫原
Synthetic peptide corresponding to Human SQSTM1/ p62 aa 157-251 (internal sequence). Carrier-protein conjugated synthetic peptide.
Database link: Q13501 -
ポジティブ・コントロール
- ICC/IF: HeLa cells treated with 50µM Chloroquine for 24 hr, HepG2 cells treated with 3µM thapsigargin 12 hrs. WB: HepG2 whole cell extracts, A549 whole cell lysate, HepG2 treated with 3µM Thapsigargin for 24 hours whole cell extracts, PC-12 whole cell lysate/extract, Rat2 whole cell lysate/extract. IP: HeLa whole cell extracts. IHC-P: human ovarian carcinoma tissue ICC/IF: treatment with bafilomycin A1 (ab120497) at 10nM-1uM.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab155686の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
1/100 - 1/1000.
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Flow Cyt |
1/50 - 1/200.
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IP | (1) |
1/100 - 1/500.
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WB | (1) |
1/500 - 1/3000. Predicted molecular weight: 47 kDa.
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ICC/IF |
1/100 - 1/1000.
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特記事項 |
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IHC-P
1/100 - 1/1000. |
Flow Cyt
1/50 - 1/200. |
IP
1/100 - 1/500. |
WB
1/500 - 1/3000. Predicted molecular weight: 47 kDa. |
ICC/IF
1/100 - 1/1000. |
ターゲット情報
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機能
Adapter protein which binds ubiquitin and may regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. May play a role in titin/TTN downstream signaling in muscle cells. May regulate signaling cascades through ubiquitination. Adapter that mediates the interaction between TRAF6 and CYLD (By similarity). May be involved in cell differentiation, apoptosis, immune response and regulation of K(+) channels. -
組織特異性
Ubiquitously expressed. -
関連疾患
Defects in SQSTM1 are a cause of Paget disease of bone (PDB) [MIM:602080]. PDB is a metabolic bone disease affecting the axial skeleton and characterized by focal areas of increased and disorganized bone turn-over due to activated osteoclasts. Manifestations of the disease include bone pain, deformity, pathological fractures, deafness, neurological complications and increased risk of osteosarcoma. PDB is a chronic disease affecting 2 to 3% of the population above the age of 40 years. -
配列類似性
Contains 1 OPR domain.
Contains 1 UBA domain.
Contains 1 ZZ-type zinc finger. -
ドメイン
The UBA domain binds specifically 'Lys-63'-linked polyubiquitin chains of polyubiquitinated substrates. Mediates the interaction with TRIM55.
The OPR domain mediates homooligomerization and interactions with PRKCZ, PRKCI, MAP2K5 and NBR1.
The ZZ-type zinc finger mediates the interaction with RIPK1. -
翻訳後修飾
Phosphorylated. May be phosphorylated by PRKCZ (By similarity). Phosphorylated in vitro by TTN. -
細胞内局在
Cytoplasm. Late endosome. Nucleus. Sarcomere (By similarity). In cardiac muscles localizes to the sarcomeric band (By similarity). Localizes to late endosomes. May also localize to the nucleus. Accumulates in neurofibrillary tangles and in Lewy bodies of neurons from individuals with Alzheimer and Parkinson disease respectively. Enriched in Rosenthal fibers of pilocytic astrocytoma. In liver cells, accumulates in Mallory bodies associated with alcoholic hepatitis, Wilson disease, indian childhood cirrhosis and in hyaline bodies associated with hepatocellular carcinoma. - Information by UniProt
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参照データベース
- Entrez Gene: 8878 Human
- Entrez Gene: 113894 Rat
- Omim: 601530 Human
- SwissProt: Q13501 Human
- SwissProt: O08623 Rat
- Unigene: 709030 Human
- Unigene: 107103 Rat
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別名
- A170 antibody
- DMRV antibody
- EBI 3 associated protein of 60 kDa antibody
see all
画像
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ab155686 staining SQSTM1/p62 (autophagosome) in control HeLa cells (left panel) and SQSTM1/p62 in HeLa cells treated with 1uM bafilomycin A1 (ab120497) for 18hrs (right panel). The cells were fixed with methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab155686 at 1/500 dilution and ab7291 (Tubulin) at 1/1000 dilution overnight at +4°C, followed by a further incubation at room temperature for 1h with a goat secondary antibody to rabbit IgG (Alexa Fluor® 488) (ab150081) at 2 μg/ml (shown in green) and a goat secondary antibody to mouse IgG (Alexa Fluor® 594) (ab150120) at 2 μg/ml (shown in pseudo color red). Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
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All lanes : Anti-SQSTM1 / p62 antibody (ab155686) at 1/1000 dilution
Lane 1 : Untreated (–) HepG2 whole cell extracts
Lane 2 : HepG2 treated with 3µM Thapsigargin for 24 hours whole cell extracts (+)
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Rabbit IgG antibody (HRP) at 1/10000 dilution
Predicted band size: 47 kDa10% gel.
Running condition: 80V, 15min; 140V, 40min.
Transfer condition: Semi-dry, 18 V, 60min (Nitrocellulose membrane).
Blocking condition: 5% non-fat milk in TBST, RT, 60min.
Primary antibody incubation: 4°C overnight.
Secondary antibody incubation: Room temperature for 1 hour.
Washing condition: 5 ml TBST, 4 x 5min.
ECL exposure.
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Immunocytochemistry/Immunofluorescent analysis of HeLa cells mock (left) and treated with 50μM Chloroquine for 24 hr (right) labeling SQSTM1/p62 (green) with ab155686 at 1/1000 dilution. Cells were fixed with 4% paraformaldehyde at RT for 15 min. Red: Phalloidin, a F-actin marker.
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Immunoprecipitation of SQSTM1/p62 protein from HeLa whole cell extracts using 5 μg of ab155686.
Western blot analysis was performed using ab155686.
Anti-Rabbit IgG was used as a secondary reagent. -
Paraffin-embedded human ovarian carcinoma tissue stained for SQSTM1/p62 with ab155686 (1/500) in Immunohistochemical analysis. Antigen retrieval: EDTA based buffer (pH 8) for 15 minutes.
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All lanes : Anti-SQSTM1 / p62 antibody (ab155686) at 1/500 dilution
Lane 1 : HepG2 whole cell extracts
Lane 2 : SQSTM1/p62 shRNA transfected HepG2 whole cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 47 kDa10% SDS-PAGE
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4% paraformaldehyde-fixed HeLa cells stained for SQSTM1/p62 (blue) using ab155686 (1/100) in Flow Cytometry. The cells were fixed at 4°C for 5 minutes. The brown line is unlabelled sample. Acquisition of >20,000 events were collected using Argon ion laser (488nm) and 525/30 bandpass filter.
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Immunocytochemistry/ Immunofluorescence analysis of HepG2 cells treated with 3μM thapsigargin for 12 hours (right) and mock (left) labeling SQSTM1/p62 with ab155686 at 1:500 (green). Cells were fixed in ice-cold MeOH for 10 minutes, permeabilized with cooled acetone for 1 minute. Cells were co-stained with Hoechst (blue).
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All lanes : Anti-SQSTM1 / p62 antibody (ab155686) at 1/1000 dilution
Lane 1 : PC-12 whole cell lysate/extract
Lane 2 : Rat2 whole cell lysate/extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 47 kDa10% SDS-PAGE
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Anti-SQSTM1 / p62 antibody (ab155686) at 1/1000 dilution + A549 whole cell lysate at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 47 kDa
10% SDS PAGE
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (57)
ab155686 は 57 報の論文で使用されています。
- Petrusca DN et al. GFI1-Dependent Repression of SGPP1 Increases Multiple Myeloma Cell Survival. Cancers (Basel) 14:N/A (2022). PubMed: 35159039
- Alvarez-Mora MI et al. Bioenergetic and Autophagic Characterization of Skin Fibroblasts from C9orf72 Patients. Antioxidants (Basel) 11:N/A (2022). PubMed: 35740026
- Zeng Y et al. Sphk1-induced autophagy in microglia promotes neuronal injury following cerebral ischaemia-reperfusion. Eur J Neurosci 56:4287-4303 (2022). PubMed: 35766986
- Yu X et al. SQSTM1/p62 promotes miR-198 loading into extracellular vesicles and its autophagy-related secretion. Hum Cell 35:1766-1784 (2022). PubMed: 36050615
- Zhang J et al. PSPH induces cell autophagy and promotes cell proliferation and invasion in the hepatocellular carcinoma cell line Huh7 via the AMPK/mTOR/ULK1 signaling pathway. Cell Biol Int 45:305-319 (2021). PubMed: 33079432