Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (heart)
Permeabilization
Yes - 0.3% Triton X
Specification
heart
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 3% Serum and 0.3% Tween 20 in PBS
Dilution
1/100
Secondary antibody
Dilution
1/400
Name
Non-Abcam antibody was used: Alexa Fluor® 555
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555
Additional data
Additional Notes
ABTRIAL-TVUV3
Abcam response
Abcam had not tested the antibody in this application and thus can't say how it should have performed. This individual received a credit for testing the antibody in IHC-Fr as part of our AbTrial program.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Mar 02 2018